Proton Gradient-Dependent Transport of p-Glucocoumaryl Alcohol in Differentiating Xylem of Woody Plants.
Identifieur interne : 000751 ( Main/Exploration ); précédent : 000750; suivant : 000752Proton Gradient-Dependent Transport of p-Glucocoumaryl Alcohol in Differentiating Xylem of Woody Plants.
Auteurs : Taku Tsuyama [Japon] ; Yasuyuki Matsushita [Japon] ; Kazuhiko Fukushima [Japon] ; Keiji Takabe [Japon] ; Kazufumi Yazaki [Japon] ; Ichiro Kamei [Japon]Source :
- Scientific reports [ 2045-2322 ] ; 2019.
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Abstract
Lignin is a cell wall component of vascular plants crucial for survival in terrestrial environments. While p-hydroxyphenyl lignin is minor, it is considered to be localised in the outermost part of the cell wall providing strong adhesion between cells, which determines cell shape. Transport of the lignin precursor from the cytosol to the cell wall is critical to regulate temporal and spatial lignin deposition; however, little information on the transport step is available. Here, we report transport activity of p-glucocoumaryl alcohol, a precursor of p-hydroxyphenyl lignin, in a broad-leaved tree (hybrid poplar, Populus sieboldii × P. grandidentata) and a coniferous tree (Japanese cypress, Chamaecyparis obtusa). Membrane vesicles of both trees were prepared from differentiating xylem with vigorous lignification and used for transport assays. Several inhibition assays indicated that not ABC transporters but the proton gradient and V-ATPase are involved in p-glucocoumaryl alcohol transport depending on ATP. These results support the hypothesis that p-glucocoumaryl alcohol is loaded into the secretory vesicles and delivered to the cell wall by exocytosis. Furthermore, this transport mechanism was common in both poplar and Japanese cypress, strongly suggesting that p-glucocoumaryl alcohol transport in the differentiating xylem is conserved within woody plants.
DOI: 10.1038/s41598-019-45394-7
PubMed: 31222148
PubMed Central: PMC6586893
Affiliations:
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wicri:level="1"><nlm:affiliation>Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601</wicri:regionArea><wicri:noRegion>464-8601</wicri:noRegion></affiliation></author><author><name sortKey="Fukushima, Kazuhiko" sort="Fukushima, Kazuhiko" uniqKey="Fukushima K" first="Kazuhiko" last="Fukushima">Kazuhiko Fukushima</name><affiliation wicri:level="1"><nlm:affiliation>Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601</wicri:regionArea><wicri:noRegion>464-8601</wicri:noRegion></affiliation></author><author><name sortKey="Takabe, Keiji" sort="Takabe, Keiji" uniqKey="Takabe K" first="Keiji" last="Takabe">Keiji 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Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Kamei, Ichiro" sort="Kamei, Ichiro" uniqKey="Kamei I" first="Ichiro" last="Kamei">Ichiro Kamei</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192</wicri:regionArea><wicri:noRegion>889-2192</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alcohols (metabolism)</term><term>Biological Transport (MeSH)</term><term>Cupressus (metabolism)</term><term>Lignin (metabolism)</term><term>Populus (metabolism)</term><term>Protons (MeSH)</term><term>Xylem (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alcools (métabolisme)</term><term>Cupressus (métabolisme)</term><term>Lignine (métabolisme)</term><term>Populus (métabolisme)</term><term>Protons (MeSH)</term><term>Transport biologique (MeSH)</term><term>Xylème (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alcohols</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cupressus</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alcools</term><term>Cupressus</term><term>Lignine</term><term>Populus</term><term>Xylème</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term><term>Protons</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Protons</term><term>Transport biologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lignin is a cell wall component of vascular plants crucial for survival in terrestrial environments. While p-hydroxyphenyl lignin is minor, it is considered to be localised in the outermost part of the cell wall providing strong adhesion between cells, which determines cell shape. Transport of the lignin precursor from the cytosol to the cell wall is critical to regulate temporal and spatial lignin deposition; however, little information on the transport step is available. Here, we report transport activity of p-glucocoumaryl alcohol, a precursor of p-hydroxyphenyl lignin, in a broad-leaved tree (hybrid poplar, Populus sieboldii × P. grandidentata) and a coniferous tree (Japanese cypress, Chamaecyparis obtusa). Membrane vesicles of both trees were prepared from differentiating xylem with vigorous lignification and used for transport assays. Several inhibition assays indicated that not ABC transporters but the proton gradient and V-ATPase are involved in p-glucocoumaryl alcohol transport depending on ATP. These results support the hypothesis that p-glucocoumaryl alcohol is loaded into the secretory vesicles and delivered to the cell wall by exocytosis. Furthermore, this transport mechanism was common in both poplar and Japanese cypress, strongly suggesting that p-glucocoumaryl alcohol transport in the differentiating xylem is conserved within woody plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31222148</PMID><DateCompleted><Year>2020</Year><Month>10</Month><Day>27</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>27</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>06</Month><Day>20</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Proton Gradient-Dependent Transport of p-Glucocoumaryl Alcohol in Differentiating Xylem of Woody Plants.</ArticleTitle><Pagination><MedlinePgn>8900</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-019-45394-7</ELocationID><Abstract><AbstractText>Lignin is a cell wall component of vascular plants crucial for survival in terrestrial environments. While p-hydroxyphenyl lignin is minor, it is considered to be localised in the outermost part of the cell wall providing strong adhesion between cells, which determines cell shape. Transport of the lignin precursor from the cytosol to the cell wall is critical to regulate temporal and spatial lignin deposition; however, little information on the transport step is available. Here, we report transport activity of p-glucocoumaryl alcohol, a precursor of p-hydroxyphenyl lignin, in a broad-leaved tree (hybrid poplar, Populus sieboldii × P. grandidentata) and a coniferous tree (Japanese cypress, Chamaecyparis obtusa). Membrane vesicles of both trees were prepared from differentiating xylem with vigorous lignification and used for transport assays. Several inhibition assays indicated that not ABC transporters but the proton gradient and V-ATPase are involved in p-glucocoumaryl alcohol transport depending on ATP. These results support the hypothesis that p-glucocoumaryl alcohol is loaded into the secretory vesicles and delivered to the cell wall by exocytosis. Furthermore, this transport mechanism was common in both poplar and Japanese cypress, strongly suggesting that p-glucocoumaryl alcohol transport in the differentiating xylem is conserved within woody plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tsuyama</LastName><ForeName>Taku</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan. tsuyama@cc.miyazaki-u.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Matsushita</LastName><ForeName>Yasuyuki</ForeName><Initials>Y</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1357-3927</Identifier><AffiliationInfo><Affiliation>Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fukushima</LastName><ForeName>Kazuhiko</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Takabe</LastName><ForeName>Keiji</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yazaki</LastName><ForeName>Kazufumi</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Research Institute for Sustainable Humanosphere, Kyoto University, Uji, 611-0011, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kamei</LastName><ForeName>Ichiro</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>06</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000438">Alcohols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011522">Protons</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000438" MajorTopicYN="N">Alcohols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029781" MajorTopicYN="N">Cupressus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011522" MajorTopicYN="Y">Protons</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>06</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31222148</ArticleId><ArticleId IdType="doi">10.1038/s41598-019-45394-7</ArticleId><ArticleId IdType="pii">10.1038/s41598-019-45394-7</ArticleId><ArticleId IdType="pmc">PMC6586893</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><noRegion><name sortKey="Tsuyama, Taku" sort="Tsuyama, Taku" uniqKey="Tsuyama T" first="Taku" last="Tsuyama">Taku Tsuyama</name></noRegion><name sortKey="Fukushima, Kazuhiko" sort="Fukushima, Kazuhiko" uniqKey="Fukushima K" first="Kazuhiko" last="Fukushima">Kazuhiko Fukushima</name><name sortKey="Kamei, Ichiro" sort="Kamei, Ichiro" uniqKey="Kamei I" first="Ichiro" last="Kamei">Ichiro Kamei</name><name sortKey="Matsushita, Yasuyuki" sort="Matsushita, Yasuyuki" uniqKey="Matsushita Y" first="Yasuyuki" last="Matsushita">Yasuyuki Matsushita</name><name sortKey="Takabe, Keiji" sort="Takabe, Keiji" uniqKey="Takabe K" first="Keiji" last="Takabe">Keiji Takabe</name><name sortKey="Yazaki, Kazufumi" sort="Yazaki, Kazufumi" uniqKey="Yazaki K" first="Kazufumi" last="Yazaki">Kazufumi Yazaki</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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